Selectin E/CD62e anticorps (FITC)

N° du produit ABIN319450

Cet anticorps Souris Monoclonal détecte spécifiquement Selectin E/CD62e dans FACS. Il présente une réactivité envers Humain et Porc.

Aperçu rapide pour Selectin E/CD62e anticorps (FITC) (ABIN319450)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: 1-2B6

Détail du produit anti-Selectin E/CD62e anticorps (FITC)

Specificité: This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.

Réactivité croisée (Details): Also reacts to: CD62P

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Immunogène: Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 10l of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E / E-Selectin

Sujet: CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

ID gène: 6401

NCBI Accession: NP_000441

UniProt: P16109

Pathways: Thromboxane A2 Receptor Signaling